Determination of mimosine and 3,4-dihydroxypyridine in milk and plasma of goats
Title | Determination of mimosine and 3,4-dihydroxypyridine in milk and plasma of goats |
Publication Type | Journal Article |
Year of Publication | 1996 |
Authors | Puchala, R, Davis, JJ, Sahlu, T |
Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
Volume | 685 |
Issue | 2 |
Pagination | 375 - 378 |
Date Published | Jan-10-1996 |
ISSN | 03784347 |
Abstract | A simple method for determination of mimosine and 3,4-dihydroxypyridine(3,4-DHP)in plasma and milk was developed. Milk and plasma, with tyrosine as internal standard, were deproteinized using 9% trichloracetic acid and extracted with diethyl ether. Metabolites were separated by isocratic high-performance liquid chromatography, with 0.02 M ortho phosphoric acid (pH 2.5) at 0.5 ml/min and a Hypersil ODS microbore column. Mimosine, 3,4-DHP and tyrosine were detected at 275 nm. The recovery of the mimosine added to the plasma samples was 101.6±2.3% and 103.3±1.0% for milk samples. 3,4-DHP recovery for plasma samples was 101.2±0.9% and for milk samples 100.8±1.4%. The analyses yielded relative standard deviation of 2.65 and 2.82%, respectively. |
URL | http://linkinghub.elsevier.com/retrieve/pii/S0378434796002216 |
DOI | 10.1016/S0378-4347(96)00221-6 |
Short Title | Journal of Chromatography B: Biomedical Sciences and Applications |