Effects of (-) Epigallocatechin-3-gallate (EGCG) on viability of Haemonchus contortus and immunie responses in white blood cells of goats in vitro

TitleEffects of (-) Epigallocatechin-3-gallate (EGCG) on viability of Haemonchus contortus and immunie responses in white blood cells of goats in vitro
Publication TypeConference Proceedings
Year of Conference2012
AuthorsZhong, RZ, Wang, Z, Zhou, DW, Goetsch, AL, Sahlu, T
Conference NameXI International Conference on Goats
Pagination362
Abstract

Effects of (-)-Epigallocatechin-3-gallate (EGCG; a polyphenol in green tea extracts) on viability of third-stage larvae (L3) of Haemonchus contortus and cytokine gene expression in white blood cells (WBC) of goats were investigated in in vitro experiments. Approximately 20 viable L3 in 10 μl phosphate buffered saline (PBS) were delivered to each well of a 96-well culture plate containing 200 μl of PBS with EGCG at concentrations of 0, 50, 100, 250, 500, 1000, 3000, or 5000 μg/ml in triplicate. Plates were incubated in a humidified incubator with 5% CO2 and 95% O2 at 37°C. Viability of larvae was determined by microscopy at 12, 24, 48, 72, and 96 h after exposed to EGCG. Data were analyzed with a MIXED model. Viability decreased (P